姓名：陈则友
职称：研究员/教授、博士生导师、南开大学百名青年学科带头人
研究领域：环境微生物学、微生物生态学、环境生物技术
研究兴趣：
1.抗生素类污染物的环境行为过程和生态健康危害；
2.环境微生物的抗菌剂耐药性的进化、传播和风险；
3.合成生物学技术在环境污染监测和修复领域的应用。
邮箱: zeyou@nankai.edu.cn
教育景
2013.10—2016.6，美国密西根州立大学，联合培养博士，导师：Hui Li 教授
2010.9—2016.6，南京农业大学，博士，博士，导师：高彦征教授（国家杰青）
科研教学经历
2025.01至今，南开大学，环境科学与工程学院，研究员/教授
2019.02-2024.12，南开大学，环境科学与工程学院，副教授，合作导师：罗义教授 (国家杰青)
2017.12-2018.12，南京大学，环境学院，助理研究员，合作导师：谷成 教授（国家长江学者）
2016.6-2017.11，美国密西根州立大学，土壤、植物和微生物系，博士后，合作导师：Hui Li教授
2020年至今，讲授本科生课程《简明分子生物学》和《环境中的奇妙微生物》。
科研项目
1. 国家自然科学基金面上项目（42277386）, 消毒剂协同选择公共设施表面细菌产生抗生素抗性的机制和风险,53万，2023,1-2026.12（主持）。
2. 国家重点研发计划青年科学家项目（2022YFC3700216），土壤及地下水中高风险病原体污染及防控，60万，2023.1-2025.12（课题负责人）。
3. 国家重点研发计划 “场地土壤污染成因与治理技术”2020年度重点专项（2020YFC1806900），场地抗生素及抗性基因环境行为与健康风险，100万，2020.11-2024.10（子课题负责人）。
4. 国家自然科学基金面上项目（41977367），耐药性人类条件致病菌及其携带的耐药基因在土壤-植物系统中的传输规律、扩散机制及健康风险研究，61万，2020.1-2023.12（主持）。
5. 国家自然科学基金青年项目（41807483），利用全细胞微生物传感器研究四环素的典型环境行为对细菌抗性基因表达的影响及机理，25万，2019.1-2021.12（主持）。
6. 天津市自然科学基金面上项目，合成生物学手段构建环境中抗生素的原位检测技术，10万，2024.10-2027.9（主持）。
7. 江苏省自然科学基金青年项目（BK20180347），抗生素抗性基因在动物粪便-好氧堆肥-农田施用全过程中的迁移和归趋研究，20万，2018.7-2021.6（主持）。
8. 南京大学中央高校基本科研业务费专项资金资助（021114380099）。利用全细胞微生物传感器研究四环素的典型环境行为对细菌抗性基因表达的影响及机理，10万，2018.1-2018.12（主持）。
学术与社会任职
2. 1. Biocontaminant 期刊编委
2. 2. Agricultural Environment and Sustainability 青年编委
3. 3. Journal of Hazardous Materials Advances 青年编委
4. 4. Bulletin of Environmental Contamination and Toxicology (BECT) 青年编委
5. 5. Frontiers in Microbiology 专刊客座编辑
学术论著
代表性著作（#co-first author,* corresponding author）
1. Xingshuo Wang, Meilun Wang, Wei Zhang, Hui Li, James M Tiedje, Jizhong Zhou, Edward Topp, Yi Luo, Zeyou Chen*.Treatment of antibiotic-manufacturing wastewater enriches for Aeromonas veronii, a zoonotic antibiotic-resistant emerging pathogen. The ISME Journal, 2025,wraf077.
2. Rongxin Cui# , Meilun Wang# , Shengyu Yuan, Xingshuo Wang, and Zeyou Chen*. An easy-to-use and field-deployable cell-free bioreporter for environmental detection of tetracyclines and macrolides.Analytical Chemistry, 2026,doi/10.1021/acs.analchem.5c04800.
3. Meilun Wang, Xingshuo Wang, Zhiyuan Chang, Baoyue Zhang, Shengyu Yuan, Xun Qian, and Zeyou Chen*.Construction and application of a multicolor bactosensor for detecting bioavailable antibiotic mixtures in the environment. ACS Synthetic Biology, 2026,in press.
4. 王星烁,常致远,王天乐,罗义,高彦征,陈则友*.被忽视的生物污染物：环境中的耐药性真菌及其抗性基因.科学通报.2026.https://doi.org/10.1360/CSB-2025-5880.
5. Zeyou Chen#, Chenyang Zhang#, Zhanhua Zhang#, Qing Chang, Cheng Gao, Xiao Liang, Xin Nie, Long Chen, Zhi Cao, Yan Lin, Pedro JJ Alvarez, Wei Chen, Tong Zhang*, Cong-Qiang Liu. Nanomineral-fuelled chemolithoautotrophy leads to substantial mercury emission. National Science Review. 2025, nwaf581.
6. Zeyou Chen, Wei Zhang, Anping Peng, Yike Shen, Xin Jin, Robert D. Stedtfeld, Stephen A. Boyd, Brian J. Teppen, James M. Tiedje, Cheng Gu, Dongqiang Zhu, Yi Luo, Hui Li*.Bacterial Community Assembly and Antibiotic Resistance Genes in Soils Exposed to Antibiotics at Environmentally Relevant Concentrations. Environmental Microbiology. 2023. 25(8):1439-1450.
7. Lichun Yin, Xiaolong Wang, Han Xu, Bo Yin, Xingshuo Wang, Yulin Zhang, Xinyao Li, Yi Luo, Zeyou Chen*,Unrecognized Risk of Perfluorooctane Sulfonate in Promoting Conjugative Transfers of Bacterial Antibiotic Resistance Genes. Applied and Environmental Microbiology. 2023;89(9):e0053323.
8. Han Xu, Zeyou Chen *, Ruiyang Huang, Yuxiao Cui, Qiang Li, Yanhui Zhao, Xiaolong Wang, Daqing Mao, Yi Luo *.Antibiotic Resistance Genes-carrying Plasmid Spreads into the Plant Endophytic Bacteria by Soil Bacteria as Carriers. Environmental Science & Technology. 2021, 55(15)：10462-10470.
9. Xiaolong Wang#, Zeyou Chen#, Quanhua Mu, Xinyan Wu, Jingjing Zhang, Daqing Mao, Yi Luo*, Pedro J. J. Alvarez*, Ionic liquid Enriches the Antibiotic Resistome, Especially Efflux Pump Genes, Before Significantly Affecting Microbial Community Structure.Environmental Science & Technology,2020, 54, 7, 4305-4315
10. Zeyou Chen, Wei Zhang, Wang Gang, Yingjie Zhang, Yanzheng Gao*, Stephen A. Boyd, Brian J. Teppen, James M. Tiedje, Dongqiang Zhu, and Hui Li*. Bioavailability of Soil-sorbed Tetracycline to Escherichia coli under Unsaturated Conditions. Environmental Science & Technology. 2017:51:6165-6173.
11. Zeyou Chen, Yingjie Zhang, Yanzheng Gao, Stephen A. Boyd, Dongqiang Zhu, and Hui Li*. Influence of Dissolved Organic Matter on Tetracycline Bioavailability to an Antibiotic-Resistant Bacterium. Environmental Science & Technology. 2015:49: 10903−10910.
13. Lei, Liusheng, Nan Chen, Zeyou Chen*, Yirong Zhao, Huai Lin, Xi Li, Wenjin Hu, Hanhui Zhang, Jingliang Shi, and Yi Luo*. Dissemination of antibiotic resistance genes from aboveground sources to groundwater in livestock farms.Water Research.2024;256: 121584.
14. Guomin Jin, Xingshuo Wang, Rongxin Cui, Shengyu Yuan, Meilun Wang, Zeyou Chen*.Comprehensive assessment of antibiotic impacts and risk thresholds on aquatic microbiomes and resistomes.Water Research, 2025, 276, 123262.
15. Jie Hou#, Zeyou Chen#, Ju Gao, Yonglei Xie, Linyun Li, Songyan Qin, Qing Wang, Daqing Mao*, Yi Luo*, Simultaneous removal of antibiotics and antibiotic resistance genes from pharmaceutical wastewater using the combinations of up-flow anaerobic sludge bed, anoxic-oxic tank, and advanced oxidation technologies. Water Research, 2019:159:511-520.
16. Zeyou Chen, Yulin Zhang, Daqing Mao, Xiaolong Wang, and Yi Luo*. NaClO Co-selects Antibiotic and Disinfectant Resistance in Klebsiella pneumonia: Implications for the Potential Risk of Extensive Disinfectant Use during COVID-19 Pandemic. Journal of Hazardous Materials (2024): 134102.
17. Guomin Jin, Meilun Wang, Xingshuo Wang, Shengyu Yuan, Anping Peng, Zeyou Chen*.Effects of sub-inhibitory antibiotic exposure on elemental cycling genes in an aquatic microbial community.Journal of Hazardous Materials. 2025, 499, 5, 140201.
18. Zeyou Chen, Yujing Duan, Lichun Yin, Ying Chen, Yingang Xue, Xiaolong Wang, Daqing Mao, Yi Luo*. Unraveling the influence of human fecal pollution on antibiotic resistance gene levels in different receiving water bodies using crAssphage indicator gene.Journal of Hazardous Materials. 2022,130005.
19. Lichun Yin, Xingshuo Wang, Yangyang Li, Ziyin Liu, Qinyuan Mei, Zeyou Chen*.Uptake of the Plant Agriculture-used Antibiotics Oxytetracycline and Streptomycin by Cherry Radish, Effect on Plant Microbiome and the Potential Health Risk.Journal of Agricultural and Food Chemistry, 2023.71(11):4561-4570.
20. Zeyou Chen, Hui Li, Yanzheng Gao*, and Anping Peng. Removal of Phenanthrene and Acenaphthene from Aqueous Solution by Enzyme-catalyzed Phenol Coupling Reactions. Chemical Engineering Journal. 2015:265:27–33.
21. Zeyou Chen, Wei Zhang*, Luxi Yang, Robert D. Stedtfeld, Anping Peng, Cheng Gu, Stephen A. Boyd, and Hui Li. Antibiotic Resistance Genes and Bacterial Communities in Cornfield and Pasture Soils Receiving Swine and Dairy Manures. Environmental Pollution, 2019:248:947-957.
22. Shengyu Yuan, Guomin Jin, Rongxin Cui, Xingshuo Wang, Meilun Wang, Zeyou Chen*.Transmission and control strategies of antimicrobial resistance from the environment to the clinic: A holistic review. Science of The Total Environment 957 (2024): 177461.
23. Anping Peng, Hu Gao, Huimin Wang,Yi Wang, Zeyou Chen*, Influence of organic cosolvents on hexabromobenzene degradation in solution by montmorillonite-templated subnanoscale zero-valent iron. Environmental Research. 2023, 229, 115986.
24. Anping Peng, Yi Wang, Lichun Yin, Zeyou Chen*, Cheng Gu. Halide salts induced the photodegradation of a fat-burning compound 2, 4-dinitrophenol by iron-montmorillonite. Chemosphere，2022，291: 132694.
25. Yujing Duan(#), Zeyou Chen(#), Lu Tan, Xiaolong Wang, Yingang Xue, Shaopeng Wang, Qing Wang, Ranjit Das, Huai Lin, Jie Hou, Linyun Li, Daqing Mao, Yi Luo,Gut resistomes, microbiota and antibiotic residues in Chinese patients undergoing antibiotic administration and healthy individuals.Science of The Total Environment, 2020:705:135674.
26. Zeyou Chen, Lichun Yin, Wei Zhang, Anping Peng, J Brett Sallach, Yi Luo, Hui Li. NaCl salinity enhances tetracycline bioavailability to Escherichia coli on agar surfaces. Chemosphere, 2022, 134921.
27. Yanhui Zhao(#), Zeyou Chen (#). Jie Hou, Daqing Mao, Huai Lin, Yingang Xue, Yi Luo. Monitoring antibiotic resistomes and bacterial microbiomes in the aerosols from fine, hazy, and dusty weather in Tianjin, China using a developed high-volume tandem liquid impinging sampler. Science of The Total Environment 2020, 731, 139242.
28. Zeyou Chen, Hui Li, Anping Peng, and Yanzheng Gao. Oxidation of Polycyclic Aromatic Hydrocarbons by Horseradish Peroxidase in Water Containing OrganicCosolvent. Environmental Science and Pollution Research. 2014:21:10696-10705.
29. Han Xu(#), Zeyou Chen(#), Xinyan Wu, Lin Zhao, Nan Wang, Daqing Mao, Hongqiang Ren, Yi Luo. Antibiotic contamination amplifies the impact of foreign antibiotic-resistant bacteria on soil bacterial community.Science of The Total Environment, 2020:23;143693.
30. Zeyou Chen, Siyuan Zhu, Lin Zhao, Xiaolong Wang, Minmin Liang, Daqing Mao, Yi Luo, and Hongqiang Ren. Occurrence of high-risk mcr-1 gene and blaNDM-1 positive superbug in the reverse osmosis filter cartridges of the household water purifiers.Journal of Hazardous Materials Letters. 2021:2:100011.
专利：
1. 陈则友,王美仑,崔蓉馨,王星烁,袁胜煜，一种 tGmR 细菌传感器的制备方法及其产品和应用，202610197572.6；
2. 陈则友,崔蓉馨,王美仑,袁胜煜，一种基于转录因子的无细胞生物传感器及其在水环境抗生素检测中的应用，CN2025103132195；
3. 陈则友,袁胜煜,尹丽纯,王星烁,金国敏,崔蓉馨，酚酸类化合物联合抗生素在制备治疗耐药病原菌感染的药物中的应用，CN202411707649.7；
4. 陈则友,袁胜煜,王美仑,王星烁，一种同时检测土壤中吸附和脱附态抗生素生物可利用性的方法，CN202411233615.9
荣誉与奖励
1.入选南开大学百名学科带头人计划
2.南开大学青年教师教学竞赛三等奖
3.南开大学优秀硕士论文指导教师
4.南开大学优秀大学生创新科研项目指导教师
5.江苏农业科技奖—技术创新一等奖, 排名：4/9
